GenCatch
(TM) Total RNA Extraction System provides an economical method
to purify total RNA from various samples such as cultured cells,
tissues, bacteria, and yeast. A simple silica-membrane
spin-column method can isolate total RNA without phenol/chloroform
extraction and ethanol precipitation. Total RNA with a molecular
size greater than 200 nucleotides (which excludes smaller RNA
such as 5S and 5.8S RNA, and tRNA) can be isolated.
Detailed
Components
High quality spin columns, collection tubes together with
buffers and protocols.
Specifications
Miniprep
Midiprep
Maxiprep
Sample Size
Tissue
10-30
mg
100-200
mg
0.5-1
g
Cell
culture
up
to 1x 107 cells
up
to 3-7x 107 cells
up
to 2-10x 108 cells
Bacteria
up
to 1x 109 cells
up
to 1x 1010 cells
up
to 5x 1010 cells
Yeast
up
to 5x 107 cells
up
to 5x 108 cells
up
to 2x 109 cells
Yield
Tissue
up
to 45 ug
up
to 450 ug
up
to 2.5 mg
Cell
culture
up
to 30 ug
up
to 300 ug
up
to 6 mg
Bacteria/yeast
up
to 65 ug
up
to 650 ug
up
to 3 mg
Elution
Volume
200
ul
500
ul
2.5
ml
Protocol
Technical
Data
Total
RNA isolated from tissue and cultured cells using GenCatch
(TM) Total RNA Extraction System was analyzed by formaldehyde
agarose gel electrophoresis.
RT-PCR
of total RNA extracted from Hela cells with GenCatch
(TM) Total RNA Extraction System. Lane 1-5 are RT-PCR products
of the total RNA. Lane 6-10 are RT-PCR of total RNA extracted from
106, 105, 104, 103,
and 102 HeLa cells respectively.
Total
RNA isolated from tissue and cultured cells using GenCatch
(TM) Total RNA Extraction System was analyzed by formaldehyde
agarose gel electrophoresis.
RT-PCR
of total RNA extracted from Hela cells with GenCatch
(TM) Total RNA Extraction System. Lane 1-5 are RT-PCR products
of the total RNA. Lane 6-10 are RT-PCR of total RNA extracted from
106, 105, 104, 103,
and 102 HeLa cells respectively.
