(TM) Plant RNA Purification System provides a simple, fast and cost-effective
method to purify RNA Plant and fugal samples. It
comes with shearing tubes for simple and fast homogenization and
filtration of plant tissues. It uses a simple silica-membrane
spin-column method to isolate total RNA greater than 200
nucleotides while excluding smaller RNA such as 5S, 5.8S and
tRNA without phenol/chloroform
extraction and ethanol precipitation. The system also provides
additional buffer for handling plant tissue that contai sticky
secondary metabolites. RNA isolated can be used for a variety of
molecular manipulations such as: Northern blotting, RT-PCR,
PolyA+ RNA selection, cDNA synthesis, Primer
extension and in vitro translation, etc.
High quality spin columns, collection tubes together with
buffers and protocols.
tp 100 mg plant tissue
to 1 g of plant tissue
tp 100 ug
to 1 mg
agarose gel analysis of total RNA isolated from various plant
samples with GenCatch
(TM) Plant RNA Purification System. Lane 1: Tobacco; 2:
Arabidopsis; 3: Rice; 4: Tomato leaves; 5: Tomato stem; 6:
Tomato root. 2 ug of total RNA was loaded per lane.
if total RNA extracted from different tissue of tomato with GenCatch
(TM) Plant RNA Purification System. Lane 1: total RNA from
leaves; 2: total RNA from stem; 3: total RNA from root; 1 kb DNA
marker is on the left.